Bifulco J M, Schaefer F W
Environmental Monitoring Systems Laboratory, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Appl Environ Microbiol. 1993 Mar;59(3):772-6. doi: 10.1128/aem.59.3.772-776.1993.
An antibody-magnetite method was developed in order to selectively concentrate Giardia cysts from water samples. The indirect technique employed a mouse immunoglobulin G anti-Giardia antibody as a primary antibody and an anti-mouse immunoglobulin G antibody-coated magnetite particle as a secondary labeling reagent. The magnetically labeled cysts were then concentrated by high-gradient magnetic separation. Ninety percent of the seeded cysts were recovered from buffer when this method was employed. An average of 82% of the seeded cysts were recovered from water samples with various turbidities. Significantly higher cyst recoveries were obtained from water samples with turbidities below 600 nephelometric turbidity units.
为了从水样中选择性地富集贾第虫包囊，开发了一种抗体-磁铁矿方法。间接技术使用小鼠免疫球蛋白G抗贾第虫抗体作为一抗，并用抗小鼠免疫球蛋白G抗体包被的磁铁矿颗粒作为二抗标记试剂。然后通过高梯度磁分离富集磁性标记的包囊。采用该方法时，90%的接种包囊可从缓冲液中回收。从不同浊度的水样中平均回收82%的接种包囊。从浊度低于600散射浊度单位的水样中获得的包囊回收率显著更高。
