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通过速度沉降法纯化鼠贾第虫包囊。

Purification of Giardia muris cysts by velocity sedimentation.

作者信息

Sauch J F

出版信息

Appl Environ Microbiol. 1984 Aug;48(2):454-5. doi: 10.1128/aem.48.2.454-455.1984.

DOI:10.1128/aem.48.2.454-455.1984
PMID:6486790
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC241540/
Abstract

Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris and slower-sedimenting Spironucleus muris and bacteria in 1.5 h.

摘要

通过单位重力速度沉降法，从初代分离物中的粪便污染物中分离出鼠贾第虫包囊。将通过在1.0 M蔗糖上离心获得的粗分离物覆盖在密度为1.01至1.03 g/ml的Percoll密度梯度上。在1.5小时内，鼠贾第虫包囊与沉降较快的粪便残渣以及沉降较慢的鼠螺旋体和细菌得到了很好的分离。

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本文引用的文献

1
Separation of cells by velocity sedimentation.通过速度沉降法分离细胞。
J Cell Physiol. 1969 Jun;73(3):191-201. doi: 10.1002/jcp.1040730305.
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Giardiasis in the mouse: an animal model.小鼠贾第虫病：一种动物模型。
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Cell separations by counterflow centrifugation.
Methods Cell Biol. 1977;15:1-14. doi: 10.1016/s0091-679x(08)60206-x.
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Density gradients prepared from colloidal silica particles coated by polyvinylpyrrolidone (Percoll).由聚乙烯吡咯烷酮包被的胶体二氧化硅颗粒（Percoll）制备的密度梯度。
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