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Use of superparamagnetic particles for isolation of cells.

作者信息

Kronick P, Gilpin R W

出版信息

J Biochem Biophys Methods. 1986 Jan;12(1-2):73-80. doi: 10.1016/0165-022x(86)90052-7.

DOI:10.1016/0165-022x(86)90052-7
PMID:2418094
Abstract

This report describes the preparation and characterization of synthetic ferritin-like particles produced by precipitation of magnetite from a mixture of ferrous and ferric ions in the presence of dextran. The 3-nm diameter particles, containing magnetite cores surrounded by chemisorbed dextran, had a magnetization of 46.7 emu/g of iron with Mössbauer quadrupole splitting of 2 delta = 0.76 mm/s. The application of these particles as a laboratory reagent for isolation of Legionella from other water bacteria was successfully tested. A 400-fold enrichment for Legionella was obtained.

摘要

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