Planck S R, Huang X N, Robertson J E, Rosenbaum J T
Department of Ophthalmology, Casey Eye Institute, Oregon Health Sciences University, Portland 97201-4197.
Curr Eye Res. 1993 Mar;12(3):205-12. doi: 10.3109/02713689308999465.
The retinal pigment epithelium (RPE) is clinically involved in diverse ocular inflammatory diseases. Because perturbed RPE cells produce a variety of inflammatory substances, RPE cells may play an integral part in these diseases. Interleukin-1 (IL-1) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are pleiotropic cytokines with the ability to trigger numerous inflammatory responses. This report shows that cultured human RPE cells synthesize interleukin-1 beta (IL-1 beta) and GM-CSF in response to the potentially inflammatory cytokine, IL-1 alpha, but not to E. coli endotoxin. Control RPE cells made little or no mRNA or protein for either IL-1 beta or GM-CSF. Upon stimulation of the cells by IL-1 alpha, both IL-1 beta and GM-CSF mRNAs were readily apparent by 3 hours, persisted for over 24 hours, and were translated into immunologically detectable proteins. GM-CSF protein was secreted into the culture medium, whereas IL-1 beta protein remained cell associated. The IL-1 alpha-induced mRNA and protein production were inhibited by dexamethasone. These observations provide additional evidence that RPE cells are capable of playing a pivotal role during ocular inflammation.
视网膜色素上皮（RPE）在临床上与多种眼部炎症性疾病相关。由于受干扰的RPE细胞会产生多种炎性物质，RPE细胞可能在这些疾病中发挥不可或缺的作用。白细胞介素-1（IL-1）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）是具有引发多种炎症反应能力的多效性细胞因子。本报告表明，培养的人RPE细胞可响应潜在的炎性细胞因子IL-1α合成白细胞介素-1β（IL-1β）和GM-CSF，但对大肠杆菌内毒素无反应。对照RPE细胞产生的IL-1β或GM-CSF的mRNA或蛋白质很少或没有。在用IL-1α刺激细胞后，3小时时IL-1β和GM-CSF的mRNA均明显出现，持续超过24小时，并被翻译成可通过免疫检测到的蛋白质。GM-CSF蛋白分泌到培养基中，而IL-1β蛋白仍与细胞相关。地塞米松可抑制IL-1α诱导的mRNA和蛋白质产生。这些观察结果提供了额外的证据，表明RPE细胞在眼部炎症过程中能够发挥关键作用。
