Suzuki T, Kawano S, Sakai A, Hirai A, Kuroiwa T
Department of Plant Sciences, Graduate School of Science, University of Tokyo, Japan.
Genes Genet Syst. 1996 Oct;71(5):329-33. doi: 10.1266/ggs.71.329.
MtDNAs from BY-2 cells and rice root were analyzed by random amplified polymorphic DNA (RAPD) assay and Southern hybridization analysis. A number of differences were observed in the RAPD patterns amplified from mtDNAs sampled at different phases of the BY-2 cell culture. RAPD fragments also varied with the template DNAs derived from various areas of rice root tip. When a RAPD fragment was hybridized to restriction fragments of whole DNAs, isolated from the distal area of the apical meristem and differentiated elongation zone of a root, two distinct stoichiometric differences were observed in the hybridization signals. This suggests that the organization of mt-genome in prototypic cells in the root apical meristem differs from that found in the differentiated cells.
通过随机扩增多态性DNA（RAPD）分析和Southern杂交分析，对来自BY - 2细胞和水稻根的线粒体DNA（MtDNA）进行了分析。在BY - 2细胞培养不同阶段采集的线粒体DNA扩增出的RAPD图谱中观察到了许多差异。RAPD片段也因源自水稻根尖不同区域的模板DNA而有所不同。当一个RAPD片段与从根尖分生组织远端区域和根的分化伸长区分离的全DNA的限制性片段杂交时，在杂交信号中观察到了两个明显的化学计量差异。这表明根尖分生组织中原型细胞的线粒体基因组组织与分化细胞中的不同。
