Bendrups A, Hilton A, Meager A, Hamilton J A
Department of Medicine, Royal Melbourne Hospital, University of Melbourne, Parkville, Australia.
Rheumatol Int. 1993;12(6):217-20. doi: 10.1007/BF00301004.
The effects of recombinant human interleukin-4 (IL-4) and the glucocorticoid, dexamethasone, on tumor necrosis factor alpha (TNF alpha) and interleukin-1 beta (IL-1 beta) levels in cultures of rheumatoid and osteoarthritic synovial tissue were studied. Low concentrations of IL-4 and dexamethasone suppressed the levels of both cytokines in the supernatants of both types of tissue after stimulation with lipopolysaccharide (LPS); the IL-1 beta and TNF alpha levels were measured by ELISA. It is suggested that it is the monocyte/macrophage in the synovial tissues that is responsive to the inhibitors. It is proposed that glucocorticoids may act on synovial tissue in this manner in vivo and IL-4 may do so if administered intraarticularly.
研究了重组人白细胞介素-4（IL-4）和糖皮质激素地塞米松对类风湿性和骨性关节炎滑膜组织培养物中肿瘤坏死因子α（TNFα）和白细胞介素-1β（IL-1β）水平的影响。低浓度的IL-4和地塞米松可抑制两种组织在脂多糖（LPS）刺激后上清液中两种细胞因子的水平；通过酶联免疫吸附测定法（ELISA）检测IL-1β和TNFα水平。提示滑膜组织中的单核细胞/巨噬细胞对这些抑制剂有反应。有人提出，糖皮质激素在体内可能以这种方式作用于滑膜组织，如果关节内注射IL-4也可能如此。
