Eriksson L, Shanwell A, Gulliksson H, Högman C F, Svensson L A, Kristensen J, Berg B
Department of Clinical Immunology and Transfusion Medicine, University Hospital, Uppsala, Sweden.
Vox Sang. 1993;64(3):133-8. doi: 10.1111/j.1423-0410.1993.tb05150.x.
Leukocyte-depleted platelet concentrates were prepared from pools of 4 buffy coats on the day after blood collection (BC-PC). The storage medium was composed of citrate phosphate dextrose plasma and a platelet-additive solution. Autologous transfusions of 111In-labelled platelets in 9 healthy volunteers were performed on the day of preparation (day 1) and on day 5. The recovery was 54.6 +/- 8.7 (day 1) and 51.9 +/- 10.4% (day 5), T1/2 was 101 +/- 28 and 61 +/- 9 h, respectively. The survival was 8.3 +/- 1.7 and 5.7 +/- 1.0 days, respectively, using linear plot, and 7.8 +/- 2.0 and 5.8 +/- 0.5 days using the multiple hit method. In a prospective clinical study a comparison of the corrected posttransfusion increments was made between BC-PCs and apheresis-PC, and between fresh (1-2 days) and stored (3-5 days) preparations. No difference was found between BC-PCs and apheresis PCs. However, fresh BC-PCs gave higher increments than stored BC-PCs. A slight numerical difference between fresh and stored apheresis-PCs was not statistically significant. It is concluded that the BC-PC method results in platelets of equal quality to apheresis-PC.
在采血后第二天，从4份 Buffy 层的混合样本中制备白细胞去除血小板浓缩物（BC - PC）。储存介质由枸橼酸盐 - 磷酸盐 - 葡萄糖血浆和血小板添加剂溶液组成。对9名健康志愿者在制备当天（第1天）和第5天进行了111In标记血小板的自体输血。回收率在第1天为54.6 +/- 8.7%，第5天为51.9 +/- 10.4%；T1/2分别为101 +/- 28小时和61 +/- 9小时。使用线性图法，生存期分别为8.3 +/- 1.7天和5.7 +/- 1.0天；使用多重打击法，生存期分别为7.8 +/- 2.0天和5.8 +/- 0.5天。在一项前瞻性临床研究中，对BC - PC和单采血小板浓缩物（apheresis - PC）之间，以及新鲜（1 - 2天）和储存（3 - 5天）制剂之间的输血后校正增加值进行了比较。未发现BC - PC和单采血小板浓缩物之间存在差异。然而，新鲜的BC - PC比储存的BC - PC有更高的增加值。新鲜和储存的单采血小板浓缩物之间的微小数值差异无统计学意义。结论是，BC - PC方法产生的血小板质量与单采血小板浓缩物相当。
