van den Brule A J, Hemrika D J, Walboomers J M, Raaphorst P, van Amstel N, Bleker O P, Meijer C J
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Fertil Steril. 1993 May;59(5):1098-104. doi: 10.1016/s0015-0282(16)55935-4.
To assess the feasibility of detecting Chlamydia trachomatis in cryopreserved donor semen by a specific, direct polymerase chain reaction (PCR).
Cryopreserved donor semen was tested for the presence of C. trachomatis by a specific PCR, directly applied to semen without prior DNA purification.
Tertiary care fertility center in a teaching hospital and university-based laboratory for molecular pathology.
Cryopreserved semen from 30 donors was investigated. These semen samples had previously given negative results in cell culture for C. trachomatis. Two different ejaculates of each donor, cryopreserved with an interval of 2 years, were retrospectively analyzed.
None.
The presence of C. trachomatis as demonstrated by PCR.
In 3 of 30 donors C. trachomatis was detected in both ejaculates, whereas in 2 additional donors only one of the two samples tested positive. Additional samples from 2 positive donors, together with samples from 3 negative donors, were studied more extensively, to test the reproducibility and reliability of PCR results. All ejaculates of the donors, previously positive for C. trachomatis by PCR, indeed appeared to be positive, whereas the samples of the negative donors remained negative.
The direct PCR is a reliable, sensitive, and valuable method for detection of C. trachomatis in semen. The incidence of contamination of donor semen with C. trachomatis in the donor population in this study stresses the need for rigorous screening of donor semen before artificial insemination, preferably using a sensitive method such as the PCR.
通过特异性直接聚合酶链反应（PCR）评估在冷冻保存的供体精液中检测沙眼衣原体的可行性。
采用特异性PCR检测冷冻保存的供体精液中是否存在沙眼衣原体，该PCR直接应用于未经DNA纯化的精液。
教学医院的三级护理生育中心和大学分子病理学实验室。
对30名供体的冷冻精液进行研究。这些精液样本先前在沙眼衣原体细胞培养中结果为阴性。回顾性分析了每位供体间隔2年冷冻保存的两份不同射精样本。
无。
PCR检测显示的沙眼衣原体存在情况。
30名供体中有3名的两份射精样本均检测出沙眼衣原体，另外2名供体中仅两份样本中的一份检测呈阳性。对2名阳性供体的额外样本以及3名阴性供体的样本进行了更广泛的研究，以测试PCR结果的可重复性和可靠性。所有先前通过PCR检测为沙眼衣原体阳性的供体射精样本确实均呈阳性，而阴性供体的样本仍为阴性。
直接PCR是检测精液中沙眼衣原体的可靠、灵敏且有价值的方法。本研究中供体人群的供体精液被沙眼衣原体污染的发生率强调了在人工授精前对供体精液进行严格筛查的必要性，最好使用如PCR这样的灵敏方法。
