Wolff H, Neubert U, Volkenandt M, Zöchling N, Schlüpen E M, Bezold G, Meurer M
Department of Dermatology, Ludwig-Maximilians-University, Munich, Germany.
Fertil Steril. 1994 Dec;62(6):1250-4. doi: 10.1016/s0015-0282(16)57194-5.
To compare the results obtained by four different techniques for the detection of Chlamydia trachomatis in the male genital tract.
Prospective study.
Andrology unit of a university hospital.
Male infertility patients.
Analysis of semen samples and urethral swabs for the presence of C. trachomatis by recombinant antibody-enzyme-linked immunosorbent assay (rELISA), polymerase chain reaction (PCR), antigen-enzyme immunoassay (EIA) and McCoy cell culture.
Detection of C. trachomatis.
In 57 of 205 semen samples (27.8%) immunoglobulin A-antibodies against C. trachomatis were found. In contrast, only 1 of 56 semen samples (1.8%) was positive for C. trachomatis-DNA by PCR, only 1 of 139 semen samples (0.7%) was positive by antigen-EIA, and only 4 of 173 urethral swabs (2.3%) grew C. trachomatis in cell culture.
The discrepancy of positive results found by the antibody-rELISA and direct methods for the detection of C. trachomatis indicates successful eradication of the microorganism in > 90% of antibody-positive men. Therefore, detection of antibodies against C. trachomatis in seminal plasma appears to be of limited diagnostic value.
比较四种不同技术检测男性生殖道沙眼衣原体的结果。
前瞻性研究。
大学医院男科。
男性不育患者。
通过重组抗体 - 酶联免疫吸附测定（rELISA）、聚合酶链反应（PCR）、抗原 - 酶免疫测定（EIA）和 McCoy 细胞培养分析精液样本和尿道拭子中沙眼衣原体的存在情况。
沙眼衣原体的检测。
在205份精液样本中的57份（27.8%）中发现了抗沙眼衣原体的免疫球蛋白A抗体。相比之下，56份精液样本中只有1份（1.8%）通过PCR检测沙眼衣原体DNA呈阳性，139份精液样本中只有1份（0.7%）通过抗原EIA呈阳性，173份尿道拭子中只有4份（2.3%）在细胞培养中培养出沙眼衣原体。
抗体 - rELISA法与直接检测沙眼衣原体方法所得阳性结果的差异表明，在90%以上抗体阳性的男性中该微生物已被成功清除。因此，精液中抗沙眼衣原体抗体的检测似乎诊断价值有限。
