Turpin P E, Maycroft K A, Rowlands C L, Wellington E M
Department of Biological Sciences, University of Warwick, Birmingham, UK.
J Appl Bacteriol. 1993 Apr;74(4):421-7. doi: 10.1111/j.1365-2672.1993.tb05149.x.
An enzyme-linked immunosorbent assay (ELISA) and a microwell fluorescent antibody (FA) direct count method have been developed for the monitoring of salmonellas in soil. Both methods have a minimum detection level of ca 10(6) cells per gram of soil. The FA direct count method gave a linear recovery for the inoculum range 10(6)-10(9) cells per gram of soil. When monitored by plate counts the survival of salmonellas was greater in a sterile than in a non-sterile soil. Evidence was found for the production of viable but non-culturable salmonellas in non-sterile soil; plate counts dropped rapidly with time, but FA direct counts and ELISA remained level. The salmonella cells became progressively smaller and rounder with time. Dead salmonella cells introduced into soil rapidly disappeared.
已开发出一种酶联免疫吸附测定法（ELISA）和一种微孔荧光抗体（FA）直接计数法，用于监测土壤中的沙门氏菌。两种方法的最低检测水平均为每克土壤约10⁶个细胞。FA直接计数法在每克土壤接种量为10⁶ - 10⁹个细胞的范围内呈现线性回收率。通过平板计数监测时，沙门氏菌在无菌土壤中的存活率高于非无菌土壤。有证据表明在非无菌土壤中会产生活的但不可培养的沙门氏菌；平板计数随时间迅速下降，但FA直接计数和ELISA结果保持稳定。随着时间的推移，沙门氏菌细胞逐渐变得更小更圆。引入土壤中的死沙门氏菌细胞迅速消失。
