Trautwein E A, Liang J, Hayes K C
Foster Biomedical Research Laboratory, Brandeis University, Waltham, Massachusetts 02254.
Lipids. 1993 Apr;28(4):305-12. doi: 10.1007/BF02536315.
Because different strains of hamsters vary in their susceptibility to gallstones, the relationship between plasma lipoproteins, hepatic cholesterol, bile lipids and bile acid profile was examined during gallstone induction in strains of male Syrian hamsters from Charles River Lakeview (CHR), Biobreeder F1B (BIO) and Harlan Sprague-Dawley (HAR). Gallstones were induced by feeding a purified diet containing 0.4 or 0.8% cholesterol for 5 wk. Basal plasma total cholesterol was similar, but the hypercholesterolemia induced by dietary challenge was significantly lower in CHR than in HAR and BIO hamsters. Cholesterol-fed CHR hamsters transported cholesterol mainly in HDL (47%), whereas VLDL-C + IDL-C predominated in BIO and HAR hamsters, and their HDL transported only 28 and 38%, respectively. HAR hamsters accumulated the most hepatic cholesterol, revealed the highest cholate/cheno ratio, the lowest glycine/taurine ratio and hydrophobicity index. HAR also developed the fewest cholesterol gallstones (23%), while 64% of CHR and 58% of BIO hamsters had cholesterol gallstones and 34% of BIO hamsters developed pigment stones. Doubling dietary cholesterol from 0.4 to 0.8% doubled the incidence of cholesterol gallstones but exerted minimal impact on other parameters compared to strain differences. Thus, different strains of hamsters vary considerably with respect to biliary cholesterol, bile acid profile and formation of cholesterol gallstones associated with differences in plasma lipoprotein profiles.
由于不同品系的仓鼠对胆结石的易感性存在差异，因此在诱导来自查尔斯河湖景（CHR）、生物育种F1B（BIO）和哈兰斯普拉格-道利（HAR）的雄性叙利亚仓鼠品系形成胆结石的过程中，研究了血浆脂蛋白、肝脏胆固醇、胆汁脂质和胆汁酸谱之间的关系。通过喂食含0.4%或0.8%胆固醇的纯化饮食5周来诱导胆结石形成。基础血浆总胆固醇相似，但饮食刺激诱导的高胆固醇血症在CHR仓鼠中显著低于HAR和BIO仓鼠。喂食胆固醇的CHR仓鼠主要通过高密度脂蛋白（HDL）转运胆固醇（47%），而极低密度脂蛋白胆固醇（VLDL-C）+中间密度脂蛋白胆固醇（IDL-C）在BIO和HAR仓鼠中占主导地位，它们的HDL分别仅转运28%和38%。HAR仓鼠肝脏中积累的胆固醇最多，胆酸盐/鹅去氧胆酸盐比率最高，甘氨酸/牛磺酸比率和疏水性指数最低。HAR仓鼠形成的胆固醇胆结石也最少（23%），而64%的CHR仓鼠和58%的BIO仓鼠有胆固醇胆结石，34%的BIO仓鼠形成色素结石。将饮食胆固醇从0.4%增加到0.8%，胆固醇胆结石的发生率翻倍，但与品系差异相比，对其他参数的影响最小。因此，不同品系的仓鼠在胆汁胆固醇、胆汁酸谱以及与血浆脂蛋白谱差异相关的胆固醇胆结石形成方面存在很大差异。
