Gargiulo A M, Pedini V, Ceccarelli P, Lorvik S
Istituto di Anatomia Normale Veterinaria, Perugia, Italy.
Anat Histol Embryol. 1995 Jun;24(2):123-6. doi: 10.1111/j.1439-0264.1995.tb00022.x.
In the present work, gustatory glands (von Ebner's glands) of the horse tongue were examined by means of five peroxidase-conjugated lectins (PNA, DBA, SBA, UEA I, WGA), with and without prior sialidase digestion, in order to investigate the presence and distribution of carbohydrate residues in secretory cells and duct cells. The most intense staining of secretory cells was observed with PNA after pre-treatment with neuraminidase. This indicates that the terminal trisaccharide sequence sialic acid- (alpha 2-->3, 6) galactosyl (beta 1-->3) N-acetylgalactosamine is the most frequent oligosaccharide chain present in glycoproteins secreted by horse gustatory glands. Secretory cells also contained oligosaccharides with terminal alpha-N-acetylgalactosamine and N-acetylglucosamine, whereas fucose was found in only a few glandular cells. The apical cytoplasm of duct lining cells reacted with all the lectins except WGA.
在本研究中，为了研究分泌细胞和导管细胞中碳水化合物残基的存在及分布情况，我们用五种过氧化物酶偶联凝集素（PNA、DBA、SBA、UEA I、WGA）对马舌的味觉腺（冯·埃布纳腺）进行了检查，检查时有的经过唾液酸酶消化，有的未经唾液酸酶消化。在用神经氨酸酶预处理后，观察到分泌细胞用PNA染色最为强烈。这表明末端三糖序列唾液酸-（α2→3，6）半乳糖基（β1→3）N-乙酰半乳糖胺是马味觉腺分泌的糖蛋白中最常见的寡糖链。分泌细胞还含有末端为α-N-乙酰半乳糖胺和N-乙酰葡糖胺的寡糖，而仅在少数腺细胞中发现了岩藻糖。除WGA外，导管内衬细胞的顶端细胞质与所有凝集素都发生反应。
