Pedini V, Ceccarelli P, Gargiulo A M
Istituto di Anatomia Normale Veterinaria, Perugia, Italy.
Res Vet Sci. 1994 Nov;57(3):353-7. doi: 10.1016/0034-5288(94)90130-9.
The glycosidic residues in the mandibular glands of five adult dogs were studied by using seven different lectin-horseradish peroxidase conjugates. In some cases a treatment with sialidase preceded the lectin staining. The mucous acinar cells contained oligosaccharides with alpha- and beta-N-acetylgalactosamine, N-acetylglucosamine and fucose residues, whereas the demilunar cells contained glycoconjugates rich in sialic acid linked to the penultimate disaccharide galactosyl-(beta 1-->3) N-acetylgalactosamine.
利用七种不同的凝集素-辣根过氧化物酶共轭物,对五只成年犬下颌腺中的糖苷残基进行了研究。在某些情况下,在凝集素染色之前先用唾液酸酶进行处理。黏液腺泡细胞含有带有α-和β-N-乙酰半乳糖胺、N-乙酰葡糖胺和岩藻糖残基的寡糖,而半月细胞含有富含唾液酸的糖缀合物,这些唾液酸与倒数第二个二糖半乳糖基-(β1→3)N-乙酰半乳糖胺相连。