Nakanishi I, Onoda N, Chung Y S, Maeda K, Kubo T, Kato Y, Sowa M
First Dept. of Surgery, Osaka City University Medical School.
Gan To Kagaku Ryoho. 1993 Apr;20(6):752-5.
An attempt was made to investigate the status of cell proliferation in the remnant stomach, using endoscopically biopsied specimens, with cell cycle analysis by flow cytometry (FCM) and ornithine decarboxylase (ODC) activity determination. Fifty-three partially gastrectomized patients (male 33, female 20) were examined. Their mean age was 60.5, and 22 underwent Billroth I (BI), and 30 Billroth II (B II) operation. The mean time elapsing from the initial operation was 4.0 years. Almost all specimens showed DNA diploidy by FCM. The S phase cell percentage was higher in the stomal area, especially in the B II group. Significantly higher ODC activity was recognized in the stomal mucosa especially in the B II group. A significant positive relationship was noted between postoperative duration and ODC activity in the B II group. These results suggest activated cell proliferation in the stomal area of the remnant stomach. Especially in the B II group, cell proliferation increased with time after initial operation. This activated cell proliferation may play some role in the carcinogenesis of the remnant stomach.
本研究旨在利用内镜活检标本，通过流式细胞术（FCM）进行细胞周期分析以及测定鸟氨酸脱羧酶（ODC）活性，来调查残胃中的细胞增殖状况。对53例接受部分胃切除术的患者（男性33例，女性20例）进行了检查。他们的平均年龄为60.5岁，其中22例行毕Ⅰ式（BI）手术，30例行毕Ⅱ式（BⅡ）手术。从初次手术到检查的平均时间为4.0年。几乎所有标本通过FCM显示为DNA二倍体。吻合口区的S期细胞百分比更高，尤其是在BⅡ组。在吻合口黏膜中，尤其是BⅡ组，ODC活性显著更高。在BⅡ组中，术后持续时间与ODC活性之间存在显著的正相关关系。这些结果表明残胃吻合口区存在细胞增殖激活现象。尤其是在BⅡ组中，初次手术后细胞增殖随时间增加。这种激活的细胞增殖可能在残胃癌变中发挥一定作用。
