Wolfe R S, Penning N
Appl Environ Microbiol. 1977 Feb;33(2):427-33. doi: 10.1128/aem.33.2.427-433.1977.
A small spirillum, designated 5175, was isolated from an anaerobic enrichment culture for Desulfuromonas in which the major medium constituents were acetate and elemental sulfur. The organisms grew only under anaerobic or microaerophilic conditions. Elemental sulfur was formed anaerobically in a malate-sulfide medium, and cell densities of 10(8) cells/ml were obtained. Hydrogen and formate were actively oxidized as substrates for growth under anaerobic conditions; S0, S032-, or S2O32-, but not SO42-, served as electron acceptors and were stoichiometrically reduced to sulfide. Malate or fumarate likewise served as electron acceptors and were reduced to succinate. Nutritional requirements were simple, no vitamins or amino acids being required. For growth in inorganic media when carbon dioxide was the only carbon source, the addition of acetate was required as a source of cell carbon. The organism is gram negative. Cells had a diameter of 0.5 mum and a wavelength of 5.0 mum. Cell suspensions exhibited an absorption spectrum indicative of a cytochrome with peaks in the reduced form at 552, 523, and 416 nm. Well growing syntrophic cultures with Chlorobium were established with formate as the substrate.
从用于脱硫单胞菌的厌氧富集培养物中分离出一种小螺旋体，命名为5175，该培养物的主要培养基成分是乙酸盐和元素硫。这些微生物仅在厌氧或微需氧条件下生长。在苹果酸 - 硫化物培养基中厌氧形成元素硫，细胞密度可达10⁸个细胞/毫升。在厌氧条件下，氢气和甲酸盐作为生长底物被积极氧化；单质硫（S⁰）、亚硫酸盐（SO₃²⁻）或硫代硫酸盐（S₂O₃²⁻），而不是硫酸盐（SO₄²⁻），作为电子受体并按化学计量还原为硫化物。苹果酸或富马酸同样作为电子受体并被还原为琥珀酸。营养需求简单，不需要维生素或氨基酸。当二氧化碳是唯一碳源时，在无机培养基中生长需要添加乙酸盐作为细胞碳源。该生物体为革兰氏阴性。细胞直径为0.5微米，波长为5.0微米。细胞悬液呈现出一种细胞色素的吸收光谱，还原形式在552、523和416纳米处有峰值。以甲酸盐为底物建立了与绿菌属生长良好的互营培养物。
