大鼠膈肌在体外释放丙氨酸的情况。
The release of alanine by rat diaphragm muscle in vitro.
作者信息
Snell K, Duff D A
出版信息
Biochem J. 1977 Feb 15;162(2):399-403. doi: 10.1042/bj1620399.
Abstract
Alanine release by rat diaphragm muscle in vitro is stimulated by glutamate, valine, leucine and glucose. The stimulation by glutamate and valine (but not leucine) is inhibited by 3-mercaptopicolinate. These results suggest a metabolic route involving phosphoenolpyruvate carboxykinase which directs amino acid carbon skeletons towards pyruvate synthesis for alanine formation.
摘要
体外培养的大鼠膈肌释放丙氨酸受到谷氨酸、缬氨酸、亮氨酸和葡萄糖的刺激。谷氨酸和缬氨酸（而非亮氨酸）的刺激作用受到3-巯基吡啶甲酸盐的抑制。这些结果提示了一条涉及磷酸烯醇式丙酮酸羧激酶的代谢途径，该途径将氨基酸碳骨架导向丙酮酸合成以形成丙氨酸。
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本文引用的文献
1
Activation of pyruvate dehydrogenase in perfused rat heart by dichloroacetate (Short Communication).
Biochem J. 1973 Jun;134(2):651-3. doi: 10.1042/bj1340651.
2
Effect of free fatty acids and insulin on protein synthesis and amino acid metabolism of isolated rat diaphragms.
Diabetes. 1967 Nov;16(11):753-64. doi: 10.2337/diab.16.11.753.
3
Hormone-fuel interrelationships during fasting.
J Clin Invest. 1966 Nov;45(11):1751-69. doi: 10.1172/JCI105481.
4
Amino acid balance across tissues of the forearm in postabsorptive man. Effects of insulin at two dose levels.
J Clin Invest. 1969 Dec;48(12):2273-82. doi: 10.1172/JCI106193.
5
The pathway of gluconeogenesis in the cortex of guinea-pig kidney. Use of aminooxyacetate as a transaminase inhibitor.
Eur J Biochem. 1972 Feb 15;25(2):366-71. doi: 10.1111/j.1432-1033.1972.tb01705.x.
6
Studies with isolated surviving rat hearts. Interdependence of free amino acids and citric-acid-cycle intermediates.
Eur J Biochem. 1973 Sep 21;38(1):86-97. doi: 10.1111/j.1432-1033.1973.tb03037.x.
7
8
Effects of dichloroacetate on the metabolism of glucose, pyruvate, acetate, 3-hydroxybutyrate and palmitate in rat diaphragm and heart muscle in vitro and on extraction of glucose, lactate, pyruvate and free fatty acids by dog heart in vivo.
Biochem J. 1973 Aug;134(4):1067-81. doi: 10.1042/bj1341067.
9
Oxidation of amino acids by diaphragms from fed and fasted rats.
Am J Physiol. 1972 Dec;223(6):1384-91. doi: 10.1152/ajplegacy.1972.223.6.1384.
10
The glucose-alanine cycle.
Metabolism. 1973 Feb;22(2):179-207. doi: 10.1016/0026-0495(73)90269-2.
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