Ruoff K L, Kuritzkes D R, Wolfson J S, Ferraro M J
Francis Blake Bacteriology Laboratories, Massachusetts General Hospital, Boston 02114.
J Clin Microbiol. 1988 Oct;26(10):2064-8. doi: 10.1128/jcm.26.10.2064-2068.1988.
Recent reports of infections with vancomycin-resistant gram-positive bacteria prompted us to study vancomycin-resistant isolates from human sources to characterize the types of bacteria displaying this phenotype. Thirty-six vancomycin-resistant gram-positive isolates, 14 from clinical specimens and 22 from stool samples, were identified. These isolates were tentatively identified as Lactobacillus spp. (25 strains), Leuconostoc spp. (6 strains), and Pediococcus spp. (3 strains) on the basis of morphology and physiological tests. Two isolates of indeterminate morphology could not be unambiguously assigned to a genus. Four isolates of vancomycin-resistant lactobacilli from normally sterile body sites were considered to be clinically significant. Vancomycin-resistant gram-positive bacteria may represent an emerging class of nosocomial pathogens. Better methods for distinguishing the various genera in the clinical microbiology laboratory are needed.
近期有关耐万古霉素革兰氏阳性菌感染的报告促使我们对来自人类样本的耐万古霉素分离株进行研究，以确定表现出这种表型的细菌类型。共鉴定出36株耐万古霉素革兰氏阳性分离株，其中14株来自临床标本，22株来自粪便样本。根据形态学和生理学测试，这些分离株初步鉴定为乳酸杆菌属（25株）、明串珠菌属（6株）和片球菌属（3株）。有两株形态不确定的分离株无法明确归为某一属。从通常无菌的身体部位分离出的4株耐万古霉素乳酸杆菌被认为具有临床意义。耐万古霉素革兰氏阳性菌可能代表一类新出现的医院病原体。临床微生物实验室需要更好的方法来区分不同的属。
