Krishnan S N, Frei E, Swain G P, Wyman R J
Department of Biology, Yale University, New Haven, Connecticut 06511.
Cell. 1993 Jun 4;73(5):967-77. doi: 10.1016/0092-8674(93)90274-t.
Passover (Pas) flies fail to jump in response to a light-off stimulus. The mutation disrupts specific synapses of the giant fibers (GFs), command neurons for this response. Pas was cloned from a P element-induced allele. The cDNA encodes a putative membrane protein of 361 amino acids. Null, hypomorphic, and dominant alleles were sequenced. In the adult central nervous system, and in the pupa during GF synapse formation, Pas is consistently expressed in the GF and in a large thoracic cell in the location of its postsynaptic targets. Pas establishes a new gene family. The Drosophila ogre protein, required for postembryonic neuroblast development, is 47% identical; the C. elegans Unc-7 protein, which when mutated alters the connectivity of a few neurons, is 33% identical.
逾越节（Pas）果蝇在光照熄灭刺激下无法跳跃。该突变破坏了巨纤维（GFs）的特定突触，而巨纤维是这种反应的指令神经元。Pas是从一个P因子诱导的等位基因中克隆出来的。该cDNA编码一个由361个氨基酸组成的假定膜蛋白。对无效、亚效和显性等位基因进行了测序。在成体中枢神经系统以及GF突触形成期间的蛹中，Pas在GF及其突触后靶点所在位置的一个大的胸细胞中持续表达。Pas建立了一个新的基因家族。果蝇中参与胚胎后期神经母细胞发育所必需的食人魔蛋白与其有47%的同源性；秀丽隐杆线虫中Unc - 7蛋白在发生突变时会改变一些神经元的连接性，它与Pas有33%的同源性。
