鸟分枝杆菌复合群MAIX AccuProbe检测阳性菌株的传统鉴定特征、分枝菌酸和脂肪酸组成及临床意义
Conventional identification characteristics, mycolate and fatty acid composition, and clinical significance of MAIX AccuProbe-positive isolates of Mycobacterium avium complex.
作者信息
Viljanen M K, Olkkonen L, Katila M L
机构信息
Mycobacterial Reference Laboratory, National Public Health Institute, Turku, Finland.
出版信息
J Clin Microbiol. 1993 May;31(5):1376-8. doi: 10.1128/jcm.31.5.1376-1378.1993.
Abstract
A total of 145 isolates belonging to the Mycobacterium avium complex (MAC) were tested with commercial acridinium ester-labeled DNA probes (AccuProbe, Gen-Probe). M. avium and M. intracellulare probes reacted with 102 and 36 isolates, respectively. The remaining seven isolates were clearly positive with the new probe, designated MAIX. Thus, the combined sensitivity of M. avium and M. intracellulare probes was 95.2%. The MAIX probe improved the sensitivity up to 100%. The MAIX probe also reacted with all M. avium (n = 20) and M. intracellulare (n = 20) isolates tested. Three of the seven MAIX-positive isolates were considered clinically significant. We conclude that the new MAIX probe should be used, in addition to M. avium and M. intracellulare probes, for the identification of MAC isolates. Our results also suggest that the probe may be used alone to cover the whole MAC when species differentiation is not required.
摘要
使用商业吖啶酯标记的DNA探针（AccuProbe，Gen-Probe）对总共145株鸟分枝杆菌复合群（MAC）菌株进行了检测。鸟分枝杆菌探针和胞内分枝杆菌探针分别与102株和36株菌株发生反应。其余7株菌株与新探针MAIX反应呈明显阳性。因此，鸟分枝杆菌探针和胞内分枝杆菌探针的联合敏感性为95.2%。MAIX探针将敏感性提高到了100%。MAIX探针也与所有测试的鸟分枝杆菌（n = 20）和胞内分枝杆菌（n = 20）菌株发生反应。7株MAIX阳性菌株中有3株被认为具有临床意义。我们得出结论，除了鸟分枝杆菌探针和胞内分枝杆菌探针外，新的MAIX探针也应用于MAC菌株的鉴定。我们的结果还表明，当不需要进行菌种区分时，该探针可单独用于覆盖整个MAC。
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