鸟分枝杆菌复合群AccuProbe阳性分离株的遗传多样性
Genetic diversity among Mycobacterium avium complex AccuProbe-positive isolates.
作者信息
Soini H, Eerola E, Viljanen M K
机构信息
Department in Turku, National Public Health Institute, Turku, Finland.
出版信息
J Clin Microbiol. 1996 Jan;34(1):55-7. doi: 10.1128/jcm.34.1.55-57.1996.
Abstract
The partial 32-kDa-protein gene sequences of 22 Mycobacterium avium complex (MAC) clinical isolates that were positive by the AccuProbe MAC probe only (not by the M. avium or M. intracellulare probe) were determined. The obtained nucleotide sequences were compared with the published sequences for M. tuberculosis, M. avium, and M. intracellulare by a sequence analysis program. There was a wide range of genetic diversity among the strains studied. Most of them (16 of 22) had sequences similar but not identical to those of M. avium and M. intracellulare. These strains were considered to be true MAC strains. Five strains had sequences in the category of the novel MAIX sequence, which was very different from the sequences of other mycobacteria analyzed thus far. In addition to these strains, one isolate had a sequence that differed greatly from the reference sequences. These results support previous findings showing that the MAC probably contains several additional species. Our results also suggest that the MAC AccuProbe may react with strains that do not belong to the MAC.
摘要
测定了22株鸟分枝杆菌复合群（MAC）临床分离株的部分32-kDa蛋白基因序列，这些分离株仅通过AccuProbe MAC探针检测呈阳性（而非通过鸟分枝杆菌或胞内分枝杆菌探针检测呈阳性）。通过序列分析程序将获得的核苷酸序列与已发表的结核分枝杆菌、鸟分枝杆菌和胞内分枝杆菌序列进行比较。在所研究的菌株中存在广泛的遗传多样性。其中大多数菌株（22株中的16株）的序列与鸟分枝杆菌和胞内分枝杆菌的序列相似但不完全相同。这些菌株被认为是真正的MAC菌株。5株菌株具有新型MAIX序列类别中的序列，该序列与迄今为止分析的其他分枝杆菌的序列非常不同。除了这些菌株外，有1株分离株的序列与参考序列有很大差异。这些结果支持了先前的研究结果，表明MAC可能包含几种其他物种。我们的结果还表明，MAC AccuProbe可能与不属于MAC的菌株发生反应。
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