Werts C, O'Callaghan D, Hofnung M, Charbit A
Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA1444, Institut Pasteur, Paris, France.
J Gen Microbiol. 1993 Apr;139(4):881-7. doi: 10.1099/00221287-139-4-881.
We have studied the immunological relatedness of LamB proteins from a wide range of enterobacterial species, using antibodies directed against denatured Escherichia coli K12 and Klebsiella pneumoniae LamB proteins (LamBE.c. and LamBK.p., respectively), and anti-peptide antibodies directed against 10 distinct loops of LamB from E. coli K12 predicted to protrude either side of the outer membrane. We have shown that a protein immunologically related to LamBE.c. and LamBK.p. was present in all members of Enterobacteriaceae tested. A protein recognized by several anti-peptide antibodies was identified in E. coli, Shigella sonnei, Salmonella typhimurium and Kleb. pneumoniae, as well as in two Citrobacter species, two Enterobacter species and Kluyvera ascorbata. The recognition patterns obtained with the anti-peptide antibodies were in agreement with the LamB protein sequence data available. They indicated that the cell surface and also the periplasmic loops of LamB are subject to great antigenic variability.
我们使用针对变性大肠杆菌K12和肺炎克雷伯菌LamB蛋白（分别为LamBE.c.和LamBK.p.）的抗体，以及针对预测位于大肠杆菌K12 LamB外膜两侧突出的10个不同环段的抗肽抗体，研究了多种肠杆菌科细菌中LamB蛋白的免疫相关性。我们发现，在所有测试的肠杆菌科成员中都存在一种与LamBE.c.和LamBK.p.免疫相关的蛋白。在大肠杆菌、宋内志贺菌、鼠伤寒沙门氏菌和肺炎克雷伯菌以及两种柠檬酸杆菌、两种肠杆菌和抗坏血酸克吕沃菌中鉴定出一种能被多种抗肽抗体识别的蛋白。用抗肽抗体获得的识别模式与现有的LamB蛋白序列数据一致。这些结果表明，LamB的细胞表面以及周质环段具有很大的抗原变异性。
