Hanlon C A, Buchanan J R, Nelson E, Niu H S, Diehl D, Rupprecht C E
Thomas Jefferson University, Department of Microbiology and Immunology, Philadelphia, PA 19107.
Rev Sci Tech. 1993 Mar;12(1):99-107. doi: 10.20506/rst.12.1.669.
During the field safety evaluation of a vaccinia-rabies glycoprotein recombinant virus vaccine for wildlife, two biomarkers were used to identify potential contact with vaccine-laden baits. Tetracycline, a commonly used and reliable calciphilic tissue marker, was included in a fish-meal polymer bait matrix and was evaluated from post-mortem bone samples. Additionally, an ante-mortem marker was needed to identify, for prospective study, raccoons which had contacted baits and thus, potentially, vaccine. Sulfadimethoxine (SDM) was included in an attractant slurry surrounding the bait, as a novel short-term seromarker. Preliminary laboratory studies in raccoons demonstrated SDM residues for up to one week following ingestion of a single 250 mg dose. During the first six days after bait distribution, 49 individual raccoons were live-trapped in the vaccination area. SDM was detectable in 38 of 49 (77.5%) serum samples. Similarly, 47 of 56 (83.9%) bone samples from raccoons collected in the vaccination area throughout the twelve-month study were tetracycline-positive. Conversely, none of the serum samples (n = 12) from the first six days of the trial nor any of the bone samples (n = 34) from raccoons in the surveillance area were biomarker-positive.
在对一种用于野生动物的痘苗-狂犬病糖蛋白重组病毒疫苗进行现场安全性评估期间，使用了两种生物标志物来确定与含疫苗诱饵的潜在接触情况。四环素是一种常用且可靠的嗜钙组织标志物，被添加到鱼粉聚合物诱饵基质中，并从死后的骨骼样本进行评估。此外，需要一种生前标志物，以便在前瞻性研究中识别接触过诱饵从而可能接触过疫苗的浣熊。磺胺二甲氧嘧啶（SDM）作为一种新型短期血清标志物，被添加到围绕诱饵的引诱剂浆液中。对浣熊进行的初步实验室研究表明，单次摄入250毫克剂量后，SDM残留可持续长达一周。在诱饵分发后的头六天里，在疫苗接种区域活捉了49只浣熊个体。在49份血清样本中的38份（77.5%）中检测到了SDM。同样，在为期十二个月的研究中，从疫苗接种区域收集的56只浣熊的骨骼样本中有47份（83.9%）四环素呈阳性。相反，试验头六天的血清样本（n = 12）以及监测区域浣熊的骨骼样本（n = 34）中均无生物标志物呈阳性。
