Yassin A F, Binder C, Schaal K P
Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, Germany.
Zentralbl Bakteriol. 1993 Feb;278(1):34-48. doi: 10.1016/s0934-8840(11)80277-x.
The mycolic acid patterns of 75 strains of mycobacteria belonging to 46 different species were studied by thin-layer chromatography (TLC). Additionally, the mycolic acid pyrolytic cleavage products were determined by capillary gas-liquid chromatography (C-GLC). Eleven different patterns based on number, type and Rf values of the detected lipid spots were identified. The mycolic acid methyl ester profiles of clinical isolates were compared with those of reference strains. In this way, it was possible to relate these clinical isolates to mycobacterial groups defined by their mycolic acid profiles. The 11 patterns and the methods used are described in detail.
通过薄层色谱法（TLC）研究了属于46个不同物种的75株分枝杆菌的分枝菌酸模式。此外，通过毛细管气液色谱法（C-GLC）测定了分枝菌酸的热解产物。根据检测到的脂质斑点的数量、类型和比移值确定了11种不同的模式。将临床分离株的分枝菌酸甲酯谱与参考菌株的进行了比较。通过这种方式，有可能将这些临床分离株与由其分枝菌酸谱定义的分枝杆菌组联系起来。详细描述了这11种模式和所使用的方法。
