Fuller T E, Mulks M H
Department of Microbiology, Michigan State University, East Lansing 48824, USA.
J Bacteriol. 1995 Dec;177(24):7265-70. doi: 10.1128/jb.177.24.7265-7270.1995.
In this paper, we report the identification, cloning, and complete nucleotide sequence of four genes from Actinobacillus pleuropneumoniae that are involved in riboflavin biosynthesis. The cloned genes can specify production of large amounts of riboflavin in Escherichia coli, can complement several defined genetic mutations in riboflavin biosynthesis in E. coli, and are homologous to riboflavin biosynthetic genes from E. coli, Haemophilus influenzae, and Bacillus subtilis. The genes have been designated A. pleuropneumoniae ribGBAH because of their similarity in both sequence and arrangement to the B. subtilis ribGBAH operon.
在本文中，我们报告了从胸膜肺炎放线杆菌中鉴定、克隆并测定了四个参与核黄素生物合成的基因的完整核苷酸序列。克隆的基因能够在大肠杆菌中大量合成核黄素，可弥补大肠杆菌核黄素生物合成中几个明确的基因突变，并且与大肠杆菌、流感嗜血杆菌和枯草芽孢杆菌的核黄素生物合成基因同源。由于这些基因在序列和排列上与枯草芽孢杆菌的ribGBAH操纵子相似，因此被命名为胸膜肺炎放线杆菌ribGBAH。
