Gomes M A, Martins M S, Costa A O, Silva E F
Departamento de Parasitologia, Universidade Federal de Minas Gerais, M.G., Brazil.
Rev Inst Med Trop Sao Paulo. 1995 May-Jun;37(3):197-200. doi: 10.1590/s0036-46651995000300003.
At this moment, the duality of species suggested for E. histolytica is being considered for discussion. In order to contribute to settling this question, we investigated the possibility of conversion of avirulent ameba to virulent ones, as well as, the possibility of increasing virulence of virulent strains, by means of association with bacteria. Five strains of E. histolytica were employed, two of them regarded as avirulent and three virulent ones. Amebas were associated with the bacteria Escherichia coli 055 and 0115, previously demonstrated as capable to modify the pathogenic behavior of E. histolytica. Changes in virulence of amebas were assessed by cytopathic effect upon cultured mammal cells and erythrophagocytosis. The virulence of pathogenic strains was significantly increased after bacteria association in opposition to what was observed for nonpathogenic ones, which were not influenced by bacteria association.
此时，关于溶组织内阿米巴所提出的物种二元性正被考虑用于讨论。为了有助于解决这个问题，我们研究了无毒力阿米巴转变为有毒力阿米巴的可能性，以及通过与细菌联合增加有毒力菌株毒力的可能性。使用了五株溶组织内阿米巴，其中两株被视为无毒力的，三株是有毒力的。将阿米巴与大肠杆菌055和0115联合，先前已证明这两种菌能够改变溶组织内阿米巴的致病行为。通过对培养的哺乳动物细胞的细胞病变效应和红细胞吞噬作用评估阿米巴毒力的变化。与非致病菌株不同，致病菌株在与细菌联合后毒力显著增加，而非致病菌株不受细菌联合的影响。
