Bellon G, Hornebeck W, Derouette J C, Robert L
Pathol Biol (Paris). 1978 Nov;26(8):515-21.
Two methods for measuring the elastase activity and the elastase inhibitory capacity of sera or other tissue extracts are described. The elastase inhibitory capacity of 40 human sera is determined using highly purified insoluble elastin in a gelified medium and this value is correlated to the alpha1-antitrypsin concentrations of these sera quantified by radial immunodiffusion. The elastase activity of these sera were determined on high molecular weight soluble kappaelastin peptides immobilized in a gelified medium and we have found a strong dispersion of the individual values. The elastase activity is not correlated either with the inhibitory capacity, the alpha1-antitrypsin or alphai-macroglobulin concentrations of these sera. The sensitivity, specificity and simplicity of these methods render them useful for their application to clinical investigations.
本文描述了两种测量血清或其他组织提取物中弹性蛋白酶活性及弹性蛋白酶抑制能力的方法。采用高度纯化的不溶性弹性蛋白在凝胶化介质中测定了40份人血清的弹性蛋白酶抑制能力，并将该值与通过放射免疫扩散法定量的这些血清中的α1-抗胰蛋白酶浓度相关联。在固定于凝胶化介质中的高分子量可溶性κ-弹性蛋白肽上测定了这些血清的弹性蛋白酶活性，我们发现个体值存在很大差异。弹性蛋白酶活性与这些血清的抑制能力、α1-抗胰蛋白酶或αi-巨球蛋白浓度均无相关性。这些方法的敏感性、特异性和简便性使其适用于临床研究。
