Kubo T, Satoh Y, Muro T, Kinoshita T, Mikami T
Laboratory of Genetic Engineering, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Curr Genet. 1995 Aug;28(3):235-41. doi: 10.1007/BF00309782.
We have constructed a complete physical map of the mitochondrial genome from the male-fertile cytoplasm of sugarbeet. The entire sequence complexity can be represented on a single circular master chromosome of 358 kb. This master chromosome contains three copies of one recombinationally active repeat sequence, with two copies in direct orientation and the other in inverted orientation. The positions of the rRNA genes and of 23 polypeptide genes, determined by filter hybridization, are scattered throughout the genome, with triplicate rrn26 genes located partially or entirely within the recombination-repeat elements. Three ribosomal-protein genes (rps1A, rps14 and rps19) were found to be absent from sugarbeet mtDNA. Our results also reveal that at least six regions homologous with cDNA are dispersed in the mitochondrial genome.
我们构建了甜菜雄性可育细胞质中线粒体基因组的完整物理图谱。整个序列复杂性可以在一条358 kb的单一环状主染色体上体现。这条主染色体包含一个重组活性重复序列的三个拷贝，其中两个拷贝呈正向排列，另一个呈反向排列。通过滤膜杂交确定的rRNA基因和23个多肽基因的位置散布于整个基因组中，三个rrn26基因部分或全部位于重组重复元件内。发现甜菜线粒体DNA中缺少三个核糖体蛋白基因（rps1A、rps14和rps19）。我们的结果还表明，至少六个与cDNA同源的区域分散在线粒体基因组中。
