Kubo T, Mikami T, Kinoshita T
Plant Breeding Institute, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Mol Gen Genet. 1993 Nov;241(3-4):479-81. doi: 10.1007/BF00284703.
From a sugar beet mitochondrial DNA library, we have isolated an open reading frame (ORF192) showing extensive homology to the gene for the 30 kDa subunit of the bovine mitochondrial complex I (NADH: ubiquinone reductase). The ORF192 was found to be actively transcribed to give an RNA of approximately 1.0 kb. We have designated this gene nad9. Transcripts from the nad9 locus are edited by five C to U transitions, increasing similarity with the amino acid sequence of the corresponding bovine and Neurospora crassa polypeptides. Southern blot hybridization also indicates that nad9 is present in the mitochondrial genomes of a variety of higher plant species.
从一个甜菜线粒体DNA文库中,我们分离出一个开放阅读框(ORF192),它与牛线粒体复合体I(NADH:泛醌还原酶)的30 kDa亚基基因具有广泛的同源性。发现ORF192被积极转录,产生一个约1.0 kb的RNA。我们将这个基因命名为nad9。nad9基因座的转录本通过五个C到U的转变进行编辑,从而增加了与相应牛和粗糙脉孢菌多肽氨基酸序列的相似性。Southern印迹杂交也表明nad9存在于多种高等植物物种的线粒体基因组中。