短小芽孢杆菌阿魏酸脱羧酶基因在大肠杆菌中的克隆、测序及表达
Cloning, sequencing, and expression in Escherichia coli of the Bacillus pumilus gene for ferulic acid decarboxylase.
作者信息
Zago A, Degrassi G, Bruschi C V
机构信息
Microbiology Group, International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
出版信息
Appl Environ Microbiol. 1995 Dec;61(12):4484-6. doi: 10.1128/aem.61.12.4484-4486.1995.
Abstract
The Bacillus pumilus gene encoding a ferulic acid decarboxylase (fdc) was identified and isolated by its ability to promote ferulic acid decarboxylation in Escherichia coli DH5 alpha. The DNA sequence of the fdc gene was determined, and the recombinant enzyme produced in E. coli was purified and characterized.
摘要
通过短小芽孢杆菌基因在大肠杆菌DH5α中促进阿魏酸脱羧的能力，鉴定并分离出了编码阿魏酸脱羧酶（fdc）的基因。测定了fdc基因的DNA序列，并对在大肠杆菌中产生的重组酶进行了纯化和特性鉴定。
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