牛血清白蛋白渗透压对浓度和pH值依赖性的分子模型。
A molecular model for the dependence of the osmotic pressure of bovine serum albumin upon concentration and pH.
作者信息
Minton A P
机构信息
Section of Physical Biochemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0830, USA.
出版信息
Biophys Chem. 1995 Dec;57(1):65-70. doi: 10.1016/0301-4622(95)00046-z.
Expressions derived from the effective hard particle model of Minton and Edelhoch (Biopolymers, 21 (1982) 451) account quantitatively for the combined data of Kanal et al. (Biophys. J., 66 (1994) 153) describing the osmotic pressure of bovine serum albumin as a function of protein concentration (< or = ca. 100 milligrams) and pH (3-8) in buffered 0.1 M NaCl. The best fit of the model yields a molar mass of 68360 and a pH-dependent value of the effective specific volume ranging from a minimum of -0.17 cm3/g at pH 4.6 (the isoelectric point) to maxima of 3.1 cm3/g at pH 3.0 and 2.2 cm3/g at pH 8.0. These values are shown to be consistent with the magnitude of known attractive and repulsive electrostatic interactions between proteins in solution.
源自明顿和埃德尔霍赫有效硬粒子模型（《生物聚合物》，21卷（1982年）451页）的表达式定量解释了卡纳尔等人（《生物物理杂志》，66卷（1994年）153页）的综合数据，这些数据描述了牛血清白蛋白在0.1M NaCl缓冲液中的渗透压与蛋白质浓度（≤约100毫克）和pH值（3 - 8）的函数关系。该模型的最佳拟合得出摩尔质量为68360，有效比容随pH值变化，在pH 4.6（等电点）时最小值为 -0.17 cm³/g，在pH 3.0时最大值为3.1 cm³/g，在pH 8.0时为2.2 cm³/g。这些值与溶液中蛋白质之间已知的吸引和排斥静电相互作用的大小一致。
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