Maskens A P, Deschner E E
J Natl Cancer Inst. 1977 May;58(5):1221-4. doi: 10.1093/jnci/58.5.1221.
A radioautographic analysis of the number and position of labeled epithelial nuclei after in vitro incorporation of tritiated thymidine ([3H]TDR) was carried out with 30 specimens of histologically normal colorectal mucosa from 26 patients, of whom 13 were being treated for rectal or sigmoid cancer and 13 were controls. A higher mean labeling index was found in the cancer than in the control group (9.8% +/- 1.2 and 7.9% +/- 0.9, respectively). Because of wide individual variations, this difference was not statistically significant. However, a highly significant upward shift of the proliferating cell compartment was observed in the cancer group, resulting in a specific modification of the [3H]TDR labeling pattern in 6 of 17 specimens. DNA synthesis predominated in the middle or middle and upper thirds of the crypts of Lieberkühn rather than in the lower third as observed in controls. The patchy alteration of epithelial cell renewal frequently accompanying colorectal cancer was suggestive of a defect in the regulatory control mechanism. This in vitro [3H]TDR incorporation assay could provide a valuable discriminatory parameter in studies of high-risk populations.
对26例患者的30份组织学正常的结直肠黏膜标本进行了放射自显影分析，以研究体外掺入氚标记胸腺嘧啶核苷（[3H]TDR）后标记上皮细胞核的数量和位置。其中13例患者正在接受直肠癌或乙状结肠癌治疗，13例为对照组。发现癌症组的平均标记指数高于对照组（分别为9.8%±1.2和7.9%±0.9）。由于个体差异较大，这种差异无统计学意义。然而，在癌症组中观察到增殖细胞区出现高度显著的上移，导致17份标本中有6份的[3H]TDR标记模式发生特定改变。与对照组不同，DNA合成主要发生在利伯kühn隐窝的中部或中上部三分之一，而非下部三分之一。结直肠癌常伴有的上皮细胞更新的斑片状改变提示调节控制机制存在缺陷。这种体外[3H]TDR掺入试验可为高危人群的研究提供有价值的鉴别参数。
