Zitzer A, Walev I, Palmer M, Bhakdi S
Institute of Medical Microbiology and Hygiene, Mainz, Germany.
Med Microbiol Immunol. 1995 May;184(1):37-44. doi: 10.1007/BF00216788.
V. cholerae El Tor cytolysin is a secreted, water-soluble protein of M(r) 60,000 that may be relevant to the pathogenesis of acute diarrhea. In this communication, we demonstrate that the toxin binds to and oligomerizes in target membranes to form SDS-stable aggregates of M(r) 200,000-250,000 that generate small transmembrane pores. Pores formed in erythrocytes were approximately 0.7 nm in size, as demonstrated by osmotic protection experiments. Binding was shown to occur in a temperature-independent manner preceding the temperature-dependent oligomerization step. Pores were also shown to be formed in L929 and HEp-2 cells, human fibroblasts and keratinocytes, albeit with highly varying efficacy. At neutral pH and in the presence of serum, human fibroblasts were able to repair a limited number of lesions. The collective data identify V. cholerae El Tor cytolysin as an oligomerizing toxin that damages cells by creating small transmembrane pores.
霍乱弧菌埃尔托溶细胞素是一种分泌型、水溶性蛋白质，分子量为60,000，可能与急性腹泻的发病机制有关。在本通讯中，我们证明该毒素可与靶膜结合并寡聚化，形成分子量为200,000 - 250,000的SDS稳定聚集体，这些聚集体可产生小的跨膜孔。通过渗透保护实验证明，在红细胞中形成的孔大小约为0.7纳米。结合显示以与温度无关的方式发生在温度依赖性寡聚化步骤之前。还显示在L929和HEp - 2细胞、人成纤维细胞和角质形成细胞中形成孔，尽管效率差异很大。在中性pH和血清存在下，人成纤维细胞能够修复有限数量的损伤。这些综合数据表明霍乱弧菌埃尔托溶细胞素是一种寡聚化毒素，通过形成小的跨膜孔来损伤细胞。
