Krishna R, Mermoud A, Baerveldt G, Minckler D S
Doheny Eye Institute, University of Southern California School of Medicine, Los Angeles, USA.
Ophthalmic Res. 1995;27(3):163-7. doi: 10.1159/000267660.
The purpose of this study was to examine whether there is a circadian rhythm of intraocular pressure (IOP) in the Lewis rat. Therefore, 20 Lewis rats (40 eyes) were subjected to an alternating 12-hour-light and 12-hour-dark cycle. Using a Tono-Pen 1, IOP was measured every 2 h between 6 a.m. and 12 midnight and every 3 h between 12 midnight and 6 a.m. for 2 consecutive days. These measurements in the Lewis rat revealed a reproducible circadian rhythm with a peak at 8 p.m. and a trough at 6 a.m. In conclusion, when using the Lewis rat as an animal model in longitudinal studies that involve the IOP, the IOP should be measured at the same time each day to correct for diurnal variations. Additionally, Lewis rats may be a useful model to study the causes of circadian rhythms of IOP and for pharmacologic studies of new glaucoma medications.
本研究的目的是检测Lewis大鼠是否存在眼压（IOP）的昼夜节律。因此，将20只Lewis大鼠（40只眼）置于12小时光照与12小时黑暗交替的周期中。使用Tono-Pen 1眼压计，在上午6点至午夜12点期间每2小时测量一次眼压，在午夜12点至上午6点期间每3小时测量一次眼压，连续测量2天。在Lewis大鼠中的这些测量结果显示出一种可重复的昼夜节律，峰值出现在晚上8点，谷值出现在上午6点。总之，在将Lewis大鼠用作涉及眼压的纵向研究的动物模型时，应每天在同一时间测量眼压，以校正昼夜变化。此外，Lewis大鼠可能是研究眼压昼夜节律成因以及新型青光眼药物药理学研究的有用模型。
