Ouviña G, Pavese A, Lemberg A, Bengochea L
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Acta Gastroenterol Latinoam. 1993;23(2):71-4.
Acetaminophen glucuronidation ability and its relationship to hepatic microsomal phospholipid changes were studied during experimental extrahepatic cholestasis. This study included two groups of Wistar rats: 1) normal rats and 2) cholestatic rats. UDP-Glucuronyltransferase activity (UDP-GT) towards acetaminophen resulted increased in 38% (p < 0.05) in the cholestatic group when compared to normal rats. We also found important changes in hepatocyte microsomal phospholipid profile. When phosphatidylcholine fatty acid composition was analysed, all of them resulted diminished except for an increment on oleic acid content (36%). This fatty acid increase in the main membrane phospholipid had a parallel behaviour with acetaminophen glucuronidation activity. This fact could support an activation role for oleic acid over this enzymic system.
在实验性肝外胆汁淤积期间，研究了对乙酰氨基酚葡萄糖醛酸化能力及其与肝微粒体磷脂变化的关系。本研究包括两组Wistar大鼠：1）正常大鼠和2）胆汁淤积大鼠。与正常大鼠相比，胆汁淤积组中对乙酰氨基酚的UDP - 葡萄糖醛酸基转移酶活性（UDP - GT）增加了38%（p < 0.05）。我们还发现肝细胞微粒体磷脂谱有重要变化。分析磷脂酰胆碱脂肪酸组成时，除油酸含量增加（36%）外，其他所有脂肪酸含量均降低。主要膜磷脂中这种脂肪酸的增加与对乙酰氨基酚葡萄糖醛酸化活性具有平行关系。这一事实可能支持油酸对该酶系统具有激活作用。
