Nachamkin I, Riddle D L, O'Connor J
Department of Pathology and Laboratory Medicine, University of Pennsylvania Medical Center, Philadelphia 19104-4283, USA.
Clin Diagn Lab Immunol. 1995 Sep;2(5):634-5. doi: 10.1128/cdli.2.5.634-635.1995.
We evaluated the Syva MicroTrak Chlamydia Blocking Antibody Assay as a confirmatory assay for the detection of Chlamydia trachomatis in urogenital specimens. During a 5-month period, 109 positives were obtained (4.9%) by the MicroTrak II Enzyme Immunoassay as implemented with the Syva XL automated enzyme immunoassay instrument. Of 98 evaluable samples, 92 (93.9%) were confirmed as positive by the blocking assay, and 3 blocking-negative samples had organisms detected by direct fluorescent-antibody analysis (blocking sensitivity, 96.8%). We found that direct fluorescent-antibody analysis of samples with a specimen-to-cutoff absorbance ratio of < or = 2.0 was a reasonable confirmation alternative and was more cost-effective than the blocking assay.
我们评估了Syva MicroTrak沙眼衣原体阻断抗体检测法，作为泌尿生殖系统标本中沙眼衣原体检测的确认检测法。在5个月的时间里，使用Syva XL自动酶免疫分析仪进行的MicroTrak II酶免疫测定法检测出109份阳性结果（4.9%）。在98份可评估样本中，92份（93.9%）通过阻断检测法确认为阳性，3份阻断检测为阴性的样本通过直接荧光抗体分析检测到病原体（阻断敏感性为96.8%）。我们发现，对标本与临界吸光度比值≤2.0的样本进行直接荧光抗体分析是一种合理的确认方法，且比阻断检测法更具成本效益。
