Mendoza M C, Martín M C, González-Hevia M A
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Epidemiol Infect. 1996 Apr;116(2):127-35. doi: 10.1017/s0950268800052353.
Ribotyping performed with six restriction endonucleases was used to study the molecular epidemiology of shigellosis in Asturias, Spain. The series included Shigella sonnei from 34 sporadic cases, 3 outbreaks and 3 reference strains, and S. Flexneri from sporadic cases and 1 reference strain. The S. sonnei strains were grouped into 5 ribotypes with Sal I, 4 with Hind III and Pvu II, 3 with Bgl II and EcoR I and 2 with Hinc II (Discriminatory Index (DI) between 0.54 and 0.14); the S. flexneri into 5 ribotypes with Sal I, Hinc II and Hind III, and 4 with the other enzymes (DI = 0.71 - 0.63). The combination of results for 2 or more enzymes facilitated and additional discrimination, the highest values in S. sonnei were for the 6 enzymes (16 types, DI = 0.91) and in S. flexneri for some combinations of 3 or more enzymes (7 types, DI = 0.81). Ribotypes with the 6 enzymes defined 16 clonal lines in S. sonnei and 7 in S. flexneri, which showed a different degree of genetic heterogeneity, and all the lines of each species falling into a different cluster. No line appeared as clearly endemic in the bowels of Asturian people.
运用六种限制性内切酶进行核糖体分型，以研究西班牙阿斯图里亚斯地区志贺氏菌病的分子流行病学。该系列研究包括来自34例散发病例、3次暴发疫情的宋内志贺氏菌以及3株参考菌株，还有来自散发病例的福氏志贺氏菌和1株参考菌株。宋内志贺氏菌菌株用Sal I可分为5个核糖体分型，用Hind III和Pvu II可分为4个，用Bgl II和EcoR I可分为3个，用Hinc II可分为2个（鉴别指数（DI）在0.54至0.14之间）；福氏志贺氏菌用Sal I、Hinc II和Hind III可分为5个核糖体分型，用其他酶可分为个4（DI = 0.71 - 0.63）。两种或更多种酶的结果组合有助于进一步鉴别，宋内志贺氏菌中6种酶的鉴别能力最强（16种类型，DI = 0.91），福氏志贺氏菌中3种或更多种酶的某些组合鉴别能力最强（7种类型，DI = 0.81）。6种酶确定的核糖体分型在宋内志贺氏菌中定义了16个克隆系，在福氏志贺氏菌中定义了7个克隆系，这些克隆系显示出不同程度的遗传异质性，且每个菌种的所有克隆系都分属于不同的簇。在阿斯图里亚斯人群肠道中没有明显的地方流行克隆系。
