通过一种改进的荧光形态计量学方法对人类粪便菌群进行表征。
Characterization of human faecal flora by means of an improved fluoro-morphometrical method.
作者信息
Jansen G J, Wilkinson M H, Deddens B, van der Waaij D
机构信息
Laboratory of Medical Microbiology, University Hospital of Groningen, The Netherlands.
出版信息
Epidemiol Infect. 1993 Oct;111(2):265-72. doi: 10.1017/s0950268800056971.
Abstract
The use of polyclonal antibodies in differentiating between samples of faecal microflora derived from ten healthy volunteers was assessed. The distribution of FITC-labelled antibodies (of the IgA, IgG and IgM isotype) over 144 morphologically distinct subsets of faecal bacteria ('morphotypes') was quantified by means of digital morphometry and quantitative immunofluorescence. Furthermore, a new dataprocessing algorithm was developed which makes objective quantitation of the antibody distributions over the faecal morphotypes possible. The results of this study imply that the antibody binding capacity of faecal morphotypes is a unique characteristic of faecal microflora.
摘要
评估了使用多克隆抗体区分来自十名健康志愿者的粪便微生物群落样本的情况。通过数字形态测量法和定量免疫荧光法对荧光素异硫氰酸酯（FITC）标记的抗体（IgA、IgG和IgM同种型）在144个形态学上不同的粪便细菌亚群（“形态型”）上的分布进行了定量。此外，还开发了一种新的数据处理算法，使得能够对粪便形态型上的抗体分布进行客观定量。这项研究的结果表明，粪便形态型的抗体结合能力是粪便微生物群落的一个独特特征。
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