Viriyakosol S, Kirkland T N
Department of Pathology, Department of Veterans Affairs Medical Center, San Diego, California 92161, USA.
Infect Immun. 1996 Feb;64(2):653-6. doi: 10.1128/iai.64.2.653-656.1996.
CD14, a glycosylphosphatidylinositol-anchored protein on the surface of monocytes, macrophages, and polymorphonuclear leukocytes, is a receptor for lipopolysaccharide (LPS). It was recently reported that an N-terminal 152-amino-acid fragment of soluble CD14 was an active soluble lipopolysaccharide receptor (T. S. -C. Juan, M. J. Kelley, D. A. Johnson, L. A. Busse, E. Hailman, S. D. Wright, and H. S. Lichenstein, J. Biol. Chem. 270:1382-1387, 1995). To determine whether the N-terminal half of the membrane CD14 was a functional LPS receptor on the cell membrane, we engineered a chimeric gene coding for amino acids 1 to 151 of CD14 fused to the C-terminal region of decay-accelerating factor and expressed it in Chinese hamster ovary cells and 70Z/3 cells. We found that the chimeric, truncated CD14 is a fully functional LPS receptor in both cell lines.
CD14是一种存在于单核细胞、巨噬细胞和多形核白细胞表面的糖基磷脂酰肌醇锚定蛋白，是脂多糖（LPS）的受体。最近有报道称，可溶性CD14的N端152个氨基酸片段是一种活性可溶性脂多糖受体（T.S.-C. Juan、M.J. Kelley、D.A. Johnson、L.A. Busse、E. Hailman、S.D. Wright和H.S. Lichenstein，《生物化学杂志》270:1382-1387，1995年）。为了确定膜CD14的N端一半是否为细胞膜上的功能性LPS受体，我们构建了一个嵌合基因，该基因编码与衰变加速因子C端区域融合的CD14的1至151个氨基酸，并在中国仓鼠卵巢细胞和70Z/3细胞中进行表达。我们发现，这种嵌合的截短型CD14在这两种细胞系中都是一种完全功能性的LPS受体。
