显性负性大鼠DNA聚合酶β突变体干扰酿酒酵母中的碱基切除修复。
Dominant negative rat DNA polymerase beta mutants interfere with base excision repair in Saccharomyces cerevisiae.
作者信息
Clairmont C A, Sweasy J B
机构信息
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
出版信息
J Bacteriol. 1996 Feb;178(3):656-61. doi: 10.1128/jb.178.3.656-661.1996.
Abstract
DNA polymerase beta is one of the smallest known eukaryotic DNA polymerases. This polymerase has been very well characterized in vitro, but its functional role in vivo has yet to be determined. Using a novel competition assay in Escherichia coli, we isolated two DNA polymerase beta dominant negative mutants. When we overexpressed the dominant negative mutant proteins in Saccharomyces cerevisiae, the cells became sensitive to methyl methanesulfonate. Interestingly, overexpression of the same polymerase beta mutant proteins did not confer sensitivity to UV damage, strongly suggesting that the mutant proteins interfere with the process of base excision repair but not nucleotide excision repair in S. cerevisiae. Our data implicate a role for polymerase IV, the S. cerevisiae polymerase beta homolog, in base excision repair in S. cerevisiae.
摘要
DNA聚合酶β是已知最小的真核生物DNA聚合酶之一。这种聚合酶在体外已得到很好的表征，但其在体内的功能作用尚未确定。利用大肠杆菌中的一种新型竞争测定法，我们分离出了两个DNA聚合酶β显性负突变体。当我们在酿酒酵母中过表达显性负突变蛋白时，细胞对甲磺酸甲酯变得敏感。有趣的是，相同的聚合酶β突变蛋白的过表达并未赋予对紫外线损伤的敏感性，这强烈表明突变蛋白干扰了酿酒酵母中的碱基切除修复过程，而不是核苷酸切除修复过程。我们的数据表明酿酒酵母中与DNA聚合酶β同源的聚合酶IV在碱基切除修复中发挥作用。
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