Wilborn F, Schmidt C A, Lorenz F, Peng R, Gelderblom H, Huhn D, Siegert W
Universitätsklinikum Rudolf Virchow, Abteilung für Innere Medizin mit Schwerpunkt Hämatologie und Onkologie, Freie Universität Berlin, Germany.
J Med Virol. 1995 Sep;47(1):65-9. doi: 10.1002/jmv.1890470113.
In order to evaluate the prevalence of human herpesvirus type 7 (HHV-7) in adult blood donors oral lavage fluid, buffy coat, and urine samples from 112 persons were examined by the polymerase chain reaction (PCR) at one time point. In addition, 11 donors were studied longitudinally over 11 weeks. When the results of the initial and the longitudinal study were combined HHV-7 DNA was found in samples from 109 of 112 (97.3%) adult blood donors. On the basis of different sensitivity levels of the first and the nested PCR differences were detected in the viral DNA load in the samples. It was found that lavage fluid regularly carried significantly higher DNA concentrations than buffy coat. Out of 112 donors, 102 (91.1%) and 8 (7.1%) were positive in the first, less sensitive PCR in lavage fluid and buffy coat, respectively (P < .0001). After nested PCR, 107 (95.5%) and 74 (66.1%) were positive in lavage fluid and buffy coat, respectively (P < .0001). Urine samples were found positive only sporadically. The longitudinal study showed that the oral lavage fluid of most of the donors consistently carried HHV-7 over up to 53 weeks, whereas buffy coat samples were positive less often. In conclusion, HHV-7 is found frequently in adult blood donors in the oral lavage fluid and buffy coat, which are, therefore, potential sources of HHV-7 transmission.
为了评估成人献血者中人类疱疹病毒7型（HHV-7）的流行情况，一次性采用聚合酶链反应（PCR）检测了112人的口腔灌洗液、血沉棕黄层和尿液样本。此外，对11名献血者进行了为期11周的纵向研究。将初始研究和纵向研究的结果相结合后发现，112名成人献血者中有109人（97.3%）的样本中检测到HHV-7 DNA。根据首次PCR和巢式PCR的不同灵敏度水平，检测到样本中病毒DNA载量存在差异。结果发现，灌洗液中的DNA浓度通常明显高于血沉棕黄层。在112名献血者中，灌洗液和血沉棕黄层在首次灵敏度较低的PCR检测中分别有102人（91.1%）和8人（7.1%）呈阳性（P < .0001）。巢式PCR后，灌洗液和血沉棕黄层分别有107人（95.5%）和74人（66.1%）呈阳性（P < .0001）。尿液样本仅偶尔呈阳性。纵向研究表明，大多数献血者的口腔灌洗液在长达53周的时间里一直携带HHV-7，而血沉棕黄层样本呈阳性的情况较少。总之，HHV-7在成人献血者的口腔灌洗液和血沉棕黄层中频繁发现，因此，它们是HHV-7传播的潜在来源。
