Wallace H L, Natelson B, Gause W, Hay J
Department of Microbiology, State University of New York at Buffalo, Buffalo, New York 14214, USA.
Clin Diagn Lab Immunol. 1999 Mar;6(2):216-23. doi: 10.1128/CDLI.6.2.216-223.1999.
We have conducted a double-blind study to assess the possible involvement of the human herpesviruses (HHVs) HHV6, HHV7, Epstein-Barr virus (EBV), and cytomegalovirus in chronic fatigue syndrome (CFS) patients compared to age-, race-, and gender-matched controls. The CFS patient population was composed of rigorously screened civilian and Persian Gulf War veterans meeting the Centers for Disease Control and Prevention's CFS case definition criteria. Healthy control civilian and veteran populations had no evidence of CFS or any other exclusionary medical or psychiatric condition. Patient peripheral blood mononuclear cells were analyzed by PCR for the presence of these HHVs. Using two-tailed Fisher's exact test analyses, we were unable to ascertain any statistically significant differences between the CFS patient and control populations in terms of the detection of one or more of these viruses. This observation was upheld when the CFS populations were further stratified with regard to the presence or absence of major axis I psychopathology and patient self-reported gradual versus acute onset of disease. In tandem, we performed serological analyses of serum anti-EBV and anti-HHV6 antibody titers and found no significant differences between the CFS and control patients.
我们进行了一项双盲研究，以评估人类疱疹病毒（HHV）HHV6、HHV7、爱泼斯坦-巴尔病毒（EBV）和巨细胞病毒与慢性疲劳综合征（CFS）患者的可能关联，并与年龄、种族和性别匹配的对照组进行比较。CFS患者群体由经过严格筛选的平民和符合疾病控制与预防中心CFS病例定义标准的海湾战争退伍军人组成。健康对照平民和退伍军人群体没有CFS或任何其他排除性医学或精神疾病的证据。通过聚合酶链反应（PCR）分析患者外周血单个核细胞中这些HHV的存在情况。使用双尾费舍尔精确检验分析，我们无法确定CFS患者和对照群体在检测一种或多种这些病毒方面存在任何统计学上的显著差异。当根据是否存在主要轴I精神病理学以及患者自我报告的疾病逐渐发作与急性发作情况对CFS群体进行进一步分层时，这一观察结果仍然成立。同时，我们对血清抗EBV和抗HHV6抗体滴度进行了血清学分析，发现CFS患者和对照患者之间没有显著差异。
