Tanaka Y, Takenaka M, Matsunaga Y, Okada S, Anan S, Yoshida H, Ra C
Department of Dermatology, Nagasaki University School of Medicine, Japan.
Arch Dermatol Res. 1995;287(8):712-7. doi: 10.1007/BF01105794.
Expression of the high affinity IgE receptor (Fc epsilon RI) on eosinophils has recently been reported. This led us to evaluate Fc epsilon RI expression on eosinophils in atopic dermatitis (AD). Double immunofluorescence stainings with an anti-Fc epsilon RI monoclonal antibody (mAb) and a polyclonal antieosinophil cationic protein (ECP) antibody were performed on lesional biopsy specimens from patients with AD and from patients with bullous pemphigoid (BP) as controls. In AD and BP lesions, 77% and 70% of eosinophils expressed Fc epsilon RI, respectively. However, the intensity of Fc epsilon RI staining in AD was much stronger than in BP, suggesting upregulation of Fc epsilon RI expression on eosinophils in AD. In addition, the eosinophils infiltrating AD lesions were stained strongly with anti-CD23 mAb and anti-IgE antibody. At the sites of mite patch testing in AD, Fc epsilon RI-, CD23- and IgE-positive eosinophils were observed to the same degree as in the lesions, and a considerable number of mite antigen-bearing eosinophils were detected. Fc epsilon RI and CD23 were both upregulated on the skin-infiltrating eosinophils in AD and bound IgE molecules.
最近有报道称嗜酸性粒细胞上存在高亲和力IgE受体（FcεRI）的表达。这促使我们评估特应性皮炎（AD）患者嗜酸性粒细胞上FcεRI的表达情况。我们使用抗FcεRI单克隆抗体（mAb）和多克隆抗嗜酸性粒细胞阳离子蛋白（ECP）抗体，对AD患者及作为对照的大疱性类天疱疮（BP）患者的皮损活检标本进行了双重免疫荧光染色。在AD和BP皮损中，分别有77%和70%的嗜酸性粒细胞表达FcεRI。然而，AD中FcεRI染色的强度远强于BP，提示AD中嗜酸性粒细胞上FcεRI表达上调。此外，浸润AD皮损的嗜酸性粒细胞用抗CD23 mAb和抗IgE抗体染色呈强阳性。在AD患者的螨斑贴试验部位，观察到FcεRI、CD23和IgE阳性的嗜酸性粒细胞与皮损中的情况相同，并且检测到相当数量携带螨抗原的嗜酸性粒细胞。在AD中，皮肤浸润的嗜酸性粒细胞上FcεRI和CD23均上调，并结合IgE分子。
