Shpakova N M, Pantaler E R, Bondarenko V A
Biokhimiia. 1995 Oct;60(10):1624-31.
The combined effect of preliminary dehydration of erythrocytes and the cationic amphiphilic compound, chlorpromazine, on the stability of cells to subsequent hyperosmotic effect, has been studied. A protective effect of chlorpromazine depending on the degree of initial cell dehydration and temperature was found. The results obtained by an addition of the cationic amphiphile at preliminary stages of the experiment and directly in the process of development of hyperosmotic lysis have been compared. A conclusion was drawn that the observed effect is not due to preliminary treatment of cells with chlorpromazine and is therefore unrelated to its influence on the regulatory systems of the cell. It has been shown also that under cold shock conditions the earlier established protective effect of chlorpromazine is also directly realized at the moment of stress exposure, i.e., with a change in temperature.
研究了红细胞初步脱水与阳离子两亲化合物氯丙嗪对细胞抵抗随后高渗效应稳定性的联合作用。发现氯丙嗪的保护作用取决于初始细胞脱水程度和温度。比较了在实验前期以及直接在高渗裂解过程中添加阳离子两亲物所获得的结果。得出的结论是，观察到的效应并非由于用氯丙嗪对细胞进行了预处理，因此与其对细胞调节系统的影响无关。还表明，在冷休克条件下，氯丙嗪较早确立的保护作用在应激暴露时刻，即温度变化时也能直接体现出来。
