Santos B R, Monteiro M G, Walzer C, Turler H, Balant L, von-Wartburg J P
Departamento de Psicobiologia, Escola Paulista de Medicina, São Paulo, Brasil.
Braz J Med Biol Res. 1995 May;28(5):513-8.
Self reports of flushing reaction after drinking, cutaneous sensitivity to alcohol (patch test), and genotypic determination of ADH2, ADH3, and ALDH2 were studied in 53 Brazilian volunteers of different ethnic groups. Genotypes were determined using single-strand conformation polymorphism in discontinuous buffer electrophoresis. Analysis of the results indicated several cases of a reported flushing reaction among ALDH2 1/1 individuals, while all but 2 cases of ALDH2 heterozygotes reported a flushing reaction. The latter subjects also had a negative result in the patch test. These preliminary results indicate that variability in the facial flushing reaction to alcohol seems to be a phenomenon resulting not only from the presence of a deficient ALDH2*2 allele, but also from other polymorphisms of alcohol-metabolizing enzymes.
对53名不同种族的巴西志愿者进行了饮酒后潮红反应的自我报告、酒精皮肤敏感性（斑贴试验）以及乙醇脱氢酶2（ADH2）、乙醇脱氢酶3（ADH3）和乙醛脱氢酶2（ALDH2）的基因分型测定。采用不连续缓冲液电泳中的单链构象多态性方法进行基因分型。结果分析表明，在乙醛脱氢酶2纯合子个体中，有几例报告有潮红反应，而除2例之外，所有乙醛脱氢酶2杂合子个体均报告有潮红反应。后一组受试者的斑贴试验结果也为阴性。这些初步结果表明，面部对酒精潮红反应的变异性似乎不仅是由乙醛脱氢酶2缺陷型等位基因的存在引起的，还与酒精代谢酶的其他多态性有关。
