莫那米多辛，一种新型纤维蛋白原受体拮抗剂。I. 生产、分离、表征及结构解析。

Monamidocin, a novel fibrinogen receptor antagonist. I. Production isolation, characterization and structural elucidation.

作者信息

Kamiyama T, Umino T, Itezono Y, Anzai Y, Nakayama N, Takemae A, Satoh T, Watanabe J, Yokose K

机构信息

Nippon Roche Research Center, Kamakura, Japan.

出版信息

J Antibiot (Tokyo). 1995 Nov;48(11):1221-5. doi: 10.7164/antibiotics.48.1221.

DOI:10.7164/antibiotics.48.1221

PMID:8557560

Abstract

Monamidocin, a novel fibrinogen receptor antagonist, has been isolated from the culture broth of Streptomyces sp. NR 0637 BY carbon adsorption, n-BuOH extraction, SP-Toyopearl, silica gel 60 silanised and Sephadex LH-20 column chromatographies and preparative HPLC. The molecular formula of monamidocin has been determined to be C15H22N4O4 from HRFAB-MS and 13C NMR spectral data. The structure of monamidocin has been determined to be N-[(S)-5-guanidino-2-hydroxypentanoyl]-L-phenylalanine by 2D NMR experiments.

摘要

莫那米多辛是一种新型纤维蛋白原受体拮抗剂，通过碳吸附、正丁醇萃取、SP- Toyopearl、硅胶60硅烷化和葡聚糖凝胶LH - 20柱色谱以及制备型高效液相色谱从链霉菌属NR 0637的培养液中分离得到。根据高分辨快原子轰击质谱（HRFAB-MS）和13C核磁共振光谱数据，确定莫那米多辛的分子式为C15H22N4O4。通过二维核磁共振实验确定莫那米多辛的结构为N-[(S)-5-胍基-2-羟基戊酰基]-L-苯丙氨酸。