Wentzel P, Jansson L, Eriksson U J
Department of Medical Cell Biology, University of Uppsala, Sweden.
Pediatr Res. 1995 Oct;38(4):598-606. doi: 10.1203/00006450-199510000-00021.
The uterine blood flow to individual implantation sites was evaluated in early normal and diabetic rat pregnancy, and related to maternal metabolic state, length of gestation, and embryonic outcome. The aim was to search for a possible coupling between the flow rate and embryonic development. We studied pregnant rats of a malformation-prone Sprague-Dawley strain on gestational d 9, 10, 11, and 12, a time period which roughly corresponds to postconception wk 3-6 in human gestation. The blood flow in the uterus was estimated with the aid of a microsphere technique, and the embryos were evaluated with respect to morphology and uterine position. We found increased blood flow in the uterine and decidual tissue of the pregnant diabetic animals compared with normal pregnant rats on all days studied. The blood perfusion peaked on gestational d 10, both in normal and diabetic pregnancy. The implantations tended to be fewer, whereas the resorption and malformation rates were higher, in the left horn than in the right horn. The blood flow in the uterine and decidual tissues was increased in the left horn in diabetic d 10 tissue, as well as d 12 tissues, thereby suggesting that compromised embryonic development is associated with increased rather than decreased supply of nutrients to the implantation site. These findings are in concert with previous in vitro results suggesting that enhanced oxidative stress due to increased substrate availability is an important factor in diabetic teratogenesis.
在正常和糖尿病大鼠妊娠早期，对各个着床部位的子宫血流进行了评估，并将其与母体代谢状态、妊娠时长及胚胎结局相关联。目的是探寻流速与胚胎发育之间可能存在的关联。我们研究了易发生畸形的斯普拉格-道利品系妊娠第9、10、11和12天的孕鼠，这一时间段大致相当于人类妊娠中受孕后第3至6周。借助微球技术估算子宫血流，并对胚胎的形态和子宫位置进行评估。我们发现，在所有研究日，与正常妊娠大鼠相比，糖尿病妊娠动物子宫和蜕膜组织中的血流增加。在正常和糖尿病妊娠中，妊娠第10天血流灌注均达到峰值。左侧子宫角的着床数往往较少，而吸收和畸形率则高于右侧子宫角。糖尿病妊娠第10天及第12天组织中，左侧子宫角的子宫和蜕膜组织血流增加，这表明胚胎发育受损与着床部位营养供应增加而非减少有关。这些发现与先前的体外研究结果一致，即底物可用性增加导致氧化应激增强是糖尿病致畸的一个重要因素。
