Sharma R K, Tolentino M V, Agarwal A
Department of Urology, Cleveland Clinic Foundation, Ohio 44195, USA.
Urology. 1996 Jan;47(1):77-81. doi: 10.1016/s0090-4295(99)80386-0.
Cryopreservation of semen is associated with reduced motility after thawing, resulting in a decreased pregnancy rate. Artificial stimulation of motility has been used in fresh semen samples. This study measured the effect of motility stimulants on various motion characteristics and other sperm functions using cryopreserved semen.
Frozen semen samples from healthy donors were thawed, and motility stimulants were added in vitro and incubated for 60 minutes. The percentages of motile spermatozoa in each specimen and other motion characteristics were measured. In addition, spermatozoon's viability, membrane integrity, and ability to penetrate bovine cervical mucus were studied after addition of stimulants.
Percentage motility and all other motion characteristics improved after stimulation with pentoxifylline, caffeine, or 2-deoxyadenosine. Linearity did not significantly differ in the control samples after adding any of the stimulants. Viability, membrane integrity, and penetration ability did not improve significantly and were comparable with control values.
Pentoxifylline, caffeine, and 2-deoxyadenosine can stimulate sperm motility and other motion characteristics. This may be beneficial in the cryopreservation of sperm from normal donors and oligozoospermic patients for use in assisted reproduction.
精液冷冻保存与解冻后活力降低有关，导致妊娠率下降。新鲜精液样本中已使用人工刺激活力的方法。本研究使用冷冻保存的精液测量活力刺激剂对各种运动特征和其他精子功能的影响。
将来自健康供体的冷冻精液样本解冻，在体外添加活力刺激剂并孵育60分钟。测量每个样本中活动精子的百分比和其他运动特征。此外，添加刺激剂后研究精子的活力、膜完整性以及穿透牛宫颈黏液的能力。
用己酮可可碱、咖啡因或2-脱氧腺苷刺激后，活力百分比和所有其他运动特征均有所改善。添加任何一种刺激剂后，对照样本中的线性度无显著差异。活力、膜完整性和穿透能力没有显著改善，与对照值相当。
己酮可可碱、咖啡因和2-脱氧腺苷可刺激精子活力和其他运动特征。这可能对正常供体和少精子症患者的精子冷冻保存用于辅助生殖有益。
