Morroni M, Barbatelli G, Carboni V, Sbarbati A, Cinti S
Institute of Normal Human Morphology, University of Ancona, Italy.
Anal Cell Pathol. 1995 Oct;9(3):235-41.
Electron spectroscopic imaging (ESI) is a recent tool for electron microscopic analysis which permits the recognition of the distribution of elements in a specimen, with a spatial resolution up to 5 nm. The authors performed the ESI together with X-ray microanalysis (EDX) and transmission electron microscopy to study granulomatous subcutaneous nodules in a patient who had undergone a desensitising therapy with an aluminium-containing vaccine. Aluminium was detected both by EDX and ESI, mainly in lysosomes of histiocytes containing needle-shaped material in a lucent matrix. However, ESI provided a better localization of aluminium with respect to EDX. This result suggests that the identification of aluminium-containing structures cannot be obtained only by ultrastructural morphology and underlines the utility of a microanalitycal study for a correct diagnosis in the presence of a needle-shaped deposition of dense material in lysosomes.
电子光谱成像（ESI）是一种用于电子显微镜分析的最新工具，它能够识别标本中元素的分布，空间分辨率可达5纳米。作者将ESI与X射线微分析（EDX）和透射电子显微镜结合使用，以研究一名接受含铝疫苗脱敏治疗患者的肉芽肿性皮下结节。通过EDX和ESI均检测到了铝，主要存在于含有透明基质中针状物质的组织细胞的溶酶体中。然而，相对于EDX，ESI对铝的定位更好。这一结果表明，仅通过超微结构形态无法确定含铝结构，强调了在溶酶体中存在针状致密物质沉积时，微观分析研究对于正确诊断的实用性。
