Pedersen E B, Poulsen F R, Zimmer J, Finsen B
Department of Anatomy and Cell Biology, University of Odense, Denmark.
Exp Brain Res. 1995;106(2):181-6. doi: 10.1007/BF00241113.
Embryonic mouse hippocampal tissue was grafted as tissue blocks to the hippocampal region of adult rats and the effect of two different immunosuppressive treatments compared. Immunosuppression with cyclosporin A, prednisolone and azathioprine or with cyclosporin A alone was compared with placebo treatment. Eight weeks' postgrafting medication with cyclosporin A, prednisolone and azathioprine had resulted in survival of 14 out of 15 grafts (93%), compared with 11 out of 14 (79%) in the group treated with cyclosporin A alone. Only 2 out of 13 grafts (15%) survived in placebo-treated animals. Transplants in the trimedication group displayed distinct cell and neuropil layers and only minimal cellular infiltration by leukocyte common antigen-expressing cells, whereas grafts in cyclosporin A- and placebo-treated groups were densely infiltrated. The results are discussed in relation to the need for extended immunosuppressive and antiinflammatory therapies after intracerebral grafting of histoincompatible tissues.
将胚胎小鼠海马组织作为组织块移植到成年大鼠的海马区，并比较两种不同免疫抑制治疗的效果。将环孢素A、泼尼松龙和硫唑嘌呤联合免疫抑制或单独使用环孢素A免疫抑制与安慰剂治疗进行比较。移植后用环孢素A、泼尼松龙和硫唑嘌呤进行八周药物治疗，15个移植物中有14个存活（93%），而单独用环孢素A治疗的组中，14个移植物中有11个存活（79%）。在接受安慰剂治疗的动物中，13个移植物中只有2个存活（15%）。三联药物治疗组的移植物显示出明显的细胞层和神经纤维层，表达白细胞共同抗原的细胞的细胞浸润极少，而环孢素A治疗组和安慰剂治疗组的移植物则有密集浸润。结合在脑内移植组织不相容组织后延长免疫抑制和抗炎治疗的必要性对结果进行了讨论。
