Margolick J B, Farzadegan H, Hoover D R, Saah A J
Department of Molecular Microbiology and Immunology, Johns Hopkins University School of Hygiene and Public Health, Baltimore 21205, USA.
J Infect Dis. 1996 Feb;173(2):468-71. doi: 10.1093/infdis/173.2.468.
The relationship between cell-associated infectious human immunodeficiency virus type 1 (HIV) load (infectious units/10(6) peripheral blood mononuclear cells [IUPM]) and phenotypes of CD4+ and CD8+ lymphocytes was studied. IUPM were measured in 242 HIV-infected homosexual men by quantitative microculture and T cell subsets by two-color flow cytometry. In multivariate analysis, IUPM correlated negatively with CD4+ lymphocyte level and with a diagnosis of AIDS and positively with the proportion of CD8+ lymphocytes expressing the activation marker CD38. After adjusting for level of CD4+ lymphocytes, men with AIDS had significantly lower IUPM than those without AIDS. The correlation between IUPM and CD4+ lymphocyte level was largely explained by correlation with level of CD4+ lymphocytes with resting phenotypes (HLA-DR-, CD38-) rather than with those expressing HLA-DR and CD38. Thus, subsets of CD4+ lymphocytes may vary in cell-associated infectious HIV content at different stages of HIV infection.
研究了细胞相关的感染性人类免疫缺陷病毒1型（HIV）载量（感染单位/10⁶外周血单个核细胞[IUPM]）与CD4⁺和CD8⁺淋巴细胞表型之间的关系。通过定量微量培养法对242名感染HIV的同性恋男性的IUPM进行了检测，并通过双色流式细胞术检测了T细胞亚群。在多变量分析中，IUPM与CD4⁺淋巴细胞水平以及艾滋病诊断呈负相关，与表达活化标志物CD38的CD8⁺淋巴细胞比例呈正相关。在对CD4⁺淋巴细胞水平进行校正后，患有艾滋病的男性的IUPM显著低于未患艾滋病的男性。IUPM与CD4⁺淋巴细胞水平之间的相关性很大程度上是由与具有静止表型（HLA-DR⁻、CD38⁻）的CD4⁺淋巴细胞水平的相关性所解释，而非与表达HLA-DR和CD38的CD4⁺淋巴细胞的相关性。因此，在HIV感染的不同阶段，CD4⁺淋巴细胞亚群的细胞相关感染性HIV含量可能会有所不同。
